FT-IR and GC–MS analyses and antioxidant activities of extracts of the wild type Saussurea sordida and Saussurea alpina native to Kazakhstan
Main Article Content
Keywords
S. sordida, S. alpine, biologically active substances, antioxidant activity
Abstract
Objective: Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide un-limited opportunities for new drug leads because of the unmatched availability of chemical diversity. The objects of the study were the medicinal plant raw materials Saussurea sordida Kar&Kir and Saussurea alpina DC. collected from southern Kazakhstan during the flowering period in the spring-summer months of 2021. experimental and theoretical substantiation of the technology for obtaining phytosubstances S. sordida, S. alpina and their study of antioxidant activity. Methods: Identification of functional groups was performed using Fourier transform infrared spectroscopy (FT-IR). The component composition of extracts of two types of medicinal plant raw materials was studied using gas chromatography with mass spectrometric detection. Results: The present study aimed to analyze the bioactive constituents of the plants S. sordida and S. alpina. The ethanol extracts were subjected to phytochemical screening and Fourier transform infrared (FTIR) spectroscopy. Phytochemical screening of the extract revealed the presence of phenolic compounds. FT-IR spectroscopic analysis revealed various characteristic bands corresponding to various functional groups, such as amines, alcohols, phenols, alkanes, alkenes, and conjugated acids, in the formulation extract. FT-IR analysis of peak values for various functional compounds, such as alcohols, phenols, carboxylic acids, aldehydes, amides, amino acids, anhydrides, esters, ketones, unsaturated aliphatic, aromatics, unsaturated heterocycles, amines, nitro compounds, alkanes, alkenes and sugars. For the first time, the compositions of petroleum ether extracts from medical plants, such as Saussurea sordida and Saussurea alpine, were investigated through GC‒MS analysis. The antioxidant activity of the medicinal plants showed that the S. alpine extract had greater activity than the S. sordida extracts according to both methods (the content of antioxidants was measured in terms of quercetin and gallic acid). Conclusion: According to the results of our study, the biologically active substances of this plant relative have an antioxidant effect due to the presence of phenolic compounds in their composition.
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