Fourier transform infrared spectrometer analysis and antimicrobial screening of ethanolic extract of Operculina terpathum from cholistan desert

Main Article Content

Arslan Ashfaq
Ghazala Shaheen https://orcid.org/0000-0002-6340-020X
Anum Khawar
Qurratul Ain Jamil
Rashida Parveen
Faheem Hadi
Aymen Owais Ghauri
Jafir Hussain Shirazi
Hafiz Muhammad Asif
Tahira Shamim
Laila Sumreen
Tanveer Ali
Muhammad Akram
Raessa Noor
Amna Mehmood
Farhan Sajid

Keywords

Anti-bacterial activity, Anti-fungal activity, Agar disc diffusion method

Abstract

Aim of present study was to assess pharmacological (antioxidant, antibacterial & antifungal) potential of Operculina terpathum seeds. Ethanolic extract was prepared and its phytochemical evaluation show the different chemical compounds such as carbohydrates, phenols, tannin, flavonoids, cardiac glycosides, steroids, alkaloids and proteins. FTIR spectra showed the presence of organic acids, hydroxyl and phenolic compounds, amino groups, aliphatic compounds, functional groups such as amide, ketone, aldehyde, aromatics and halogen compounds. Antioxidant activity of the Operculina terpathum alcoholic extract was performed by DPPH method and it showed 97.13%whereas IC50±SEM (μg/ml) was 1.425±0.16. Antibacterial activity was performed against different bacterial strains and results were comparable with that of standard. Maximum antibacterial activity was exhibited by Bacillus subtillis (28.33±2 mm) and Bacillus pumilus (25.33±2 mm) respectively. Antifungal activity was also performed and it showed maximum activity against Aspergillus flavous and Candida albicans6±1, 5±1mm respectively. These results showed that Operculina terpathum has good antibacterial and an

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References

1. Munuswamy H, Thirunavukkarasu T, Rajamani S, et al. A review on antimicrobial efficacy of some traditional medicinal plants  in Tamilnadu. Journal of Acute Disease. 2013;2(2):99-105.
2. Krishna S, Uhlemann AC, Haynes RK. Artemisinins: mechanisms of action and potential for resistance. Drug Resistance Updates.2004;7(4-5):233-244.
3. da Silva BM, Eichenberger M, Ditterich RG, et al. The use of medicinal plants and their possible drug interactions with allopathic drugs in a metropolitan region of Curitiba-PR. Journal of Research in Dentistry. 2018;5(6):106-112.
4. Usman H, Kaigama AU, Ibisagba OO, et al. Phytoconstituents evaluation and antimicrobial efficacy of the crude flavonoids and saponins rootbark extracts of Terminalia avicennioides and Ficus polita. Journal of Herbmed Pharmacology. 2018;7(2).
5. Luepke KH, Suda KJ, Boucher H, et al. Past, present, and future of antibacterial economics: increasing bacterial resistance,limited antibiotic pipeline, and societal implications. Pharmacotherapy: The Journal of Human Pharmacology and DrugTherapy. 2017;37(1):71-84. https://doi.org/10.1002/phar.1868
6. Uttra AM, Ahsan H, Hasan UH, et al. Traditional medicines of plant origin used for the treatment of inflammatory disorders in Pakistan: a review. Journal of Traditional Chinese Medicine. 2018;38(4):636-656.
7. Kanwal H, Sherazi BA. Herbal medicine: Trend of practice, perspective, and limitations in Pakistan. Asian Pacific Journal of Health Sciences. 2017;4(4):6-8.
8. Prakash A, Janmeda P, Sharma V. Bioactivity and Pharmacological Potential of Trianthema portulacastrum L. (Angiosperms:Aizoaceae): An Overview. Plant Science Today. 2019;6(sp1):590-599.
9. Mensor LL, Menezes FS, Leitão GG, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001;15(2):127-130. https://doi.org/10.1002/ptr.687
10. Neenu A. Santhosh, Varghese CD. Evaluation on phytochemical, antioxidant and antibacterial potential of the root of operculina turpethum (linn.) Silva manso. World journal of pharmacy and pharmaceutical sciences. 2017;6(9):979-983.
11. Sridhar N, Duggirala SL, Puchchakayala G. Antimicrobial activity of ethanolic extracts of Justicia neesii. Bangladesh Journal of Pharmacology. 2014;9(4):624-627. https://doi.org/10.4103/0253-7613.153425
12. Chibane LB, Degraeve P, Ferhout H, et al. Plant antimicrobial polyphenols as potential natural food preservatives. Journal of the Science of Food and Agriculture. 2019;99(4):1457-1474. https://doi.org/10.1002/jsfa.9357
13. Danish P, Ali Q, Hafeez M, et al. Antifungal and antibacterial activity of aloe vera plant extract. Biological and Clinical Sciences Research Journal. 2020;2020(1):e004.
14. Pisoschi AM, Pop A, Georgescu C, et al. An overview of natural antimicrobials role in food. European Journal of Medicinal Chemistry. 2018;143:922-935. https://doi.org/10.1016/j.ejmech.2017.11.095
15. Adamczak A, Ożarowski M, Karpiński TM. Antibacterial activity of some flavonoids and organic acids widely distributed in plants. Journal of clinical medicine. 2020;9(1):109. https://doi.org/10.3390/jcm9010109
16. Shareef H, Rizwani GH, Mandukhail SR, et al. BMC Complementary and Alternative Medicine. 2014;14:479.

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