Beta2-adrenergic receptor polymorphisms among healthy Jordanian population

Main Article Content

Malek Zihlif https://orcid.org/0000-0002-8005-3908
Zainab Zakaraya https://orcid.org/0000-0003-3691-9759
Laila Habahbeh https://orcid.org/0000-0002-9829-200X
Mohammad Al Shhab https://orcid.org/0000-0003-0160-8264
Rajaa Daghashb https://orcid.org/0009-0007-2824-9448
Andi Dian PERMANA https://orcid.org/0000-0003-2168-1688
Ahmad R. Alsayed https://orcid.org/0000-0002-1324-7884

Keywords

β2-Adrenergic Receptors, healthy, jordan, pcr, polymorphis

Abstract

Background: Several polymorphisms of the β2-adrenergic receptor gene have been identified. These polymorphisms affect receptor function and significantly reduce expressed receptors in the cells. The main objective of this study is to determine the frequency of β2 adrenergic receptor polymorphism among a healthy Jordanian population and compare it with those of different ethnic groups. Blood samples were collected from 96 healthy Jordanians. β2-adrenergic receptor gene polymorphism at codon 16 and codon 27 was assessed by Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism RFLP. The minor allele frequency of β2-adrenergic receptor polymorphism at position 46 (46 G/A) was 31%, while the polymorphism at position 79 (79 C/G) was 14% in the Jordanian population. The genotype frequencies for 46 (46 G/A) were 51% for (GG), 13% for (AA) and 36% for (GA). In case 79 C/G, the genotype frequencies were 87.5% 7.3 % 5.2% CC, GG and CG genotypes, respectively. This study’s results indicate marked interethnic differences in the genotype and allele frequencies of the two β2-adrenergic receptor polymorphisms among healthy Jordanians, Caucasians, African Americans, and Asians.

Abstract 180 | PDF Downloads 95

References

1. Shin J, Johnson JA. Pharmacogenetics of beta-blockers. Pharmacotherapy 2007;27(6):874-87. https://doi.org/10.1592/phco.27.6.874
2. Skeberdis VA. Structure and function of beta3-adrenergic receptors. Medicina (Kaunas) 2004;40(5):407-13.
3. Large V, Hellström L, Reynisdottir S, Lönnqvist F, Eriksson P, Lannfelt L, et al. Human beta-2 adrenoceptor gene polymorphisms are highly frequent in obesity and associate with altered adipocyte beta-2 adrenoceptor function. J Clin Invest. 1997;100(12):3005-13.
4. de Paiva AC, Marson FA, Ribeiro JD, Bertuzzo CS. Asthma: Gln27Glu and Arg16Gly polymorphisms of the beta2-adrenergic receptor gene as risk factors. Allergy Asthma Clin Immunol. 2014;10(1):8. https://doi.org/10.1186/1710-1492-10-8
5. Ramsay CE, Hayden CM, Tiller KJ, Burton PR, Goldblatt J, Lesouef PN. Polymorphisms in the beta2-adrenoreceptor gene are associated with decreased airway responsiveness. Clin Exp Allergy. 1999;29(9):1195-203. https://doi.org/10.1046/j.1365-2222.1999.00570.x
6. Liggett SB. The pharmacogenetics of beta2-adrenergic receptors: relevance to asthma. Allergy Clin Immunol. 2000;105(2 Pt 2):S487-92. https://doi.org/10.1016/s00916749(00)90048-4
7. Turki J, Pak J, Green SA, Martin RJ, Liggett SB. Genetic polymorphisms of the beta 2-adrenergic receptor in nocturnal and nonnocturnal asthma. Evidence that Gly16 correlates with the nocturnal phenotype. J Clin Invest. 1995;95(4):1635-41. https://doi.org/10.1172/jci117838
8. Karam RA, Sabbah NA, Zidan HE, Rahman HM. Association between genetic polymorphisms of beta2 adrenergic receptors and nocturnal asthma in Egyptian children. J Investig Allergol Clin Immunol. 2013;23(4):262-6.
9. Adimi Naghan P, Fahimi F, Nadji SA, Naderi N, Soleimani F, Masjedi MR. A pilot study of polymorphism of adrenergic Beta-2receptor and mild asthma: a clinical and pharmacogenetic study. Iran J Pharm Res. 2013;12(1):199-204. http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3813221/
10. Al-Rubaish A. β2-adrenergic receptor gene polymorphisms in normal and asthmatic individuals in the Eastern Province of Saudi Arabia. Ann Saudi Med. 2011;31(6):586-90. https://doi.org/10.4103/0256-4947.87094
11. Xie HG, Stein CM, Kim RB, Xiao ZS, He N, Zhou HH, et al. Frequency of functionally important beta-2 adrenoceptor polymorphisms varies markedly among African-American, Caucasian and Chinese individuals. Pharmacogenetics. 1999;9(4):511-6.
12. Theofilogiannakos EK, Boudoulas KD, Gawronski BE, Langaee TY, Kelpis TG, Pitsis AA, et al. Beta-adrenergic receptor polymorphisms: A basis for pharmacogenetics. World Journal of Cardiovascular Diseases. 2013;3(06):406.
13. Kato N, Sugiyama T, Morita H, Kurihara H, Sato T, Yamori Y, et al. Association analysis of beta(2)-adrenergic receptor polymorphisms with hypertension in Japanese. Hypertension. 2001;37(2):286-92. https://doi.org/10.1161/01.hyp.37.2.286
14. Liggett SB. beta(2)-adrenergic receptor pharmacogenetics. Am J Respir Crit Care Med. 2000;161(3 Pt 2):S197-201. https://doi.org/10.1164/ajrccm.161.supplement_2.a1q4-10
15. Liang SQ, Chen XL, Deng JM, Wei X, Gong C, Chen ZR, et al. Beta-2 adrenergic receptor (ADRB2) gene polymorphisms and the risk of asthma: a meta-analysis of case-control studies. PLoS One. 2014;9(8):e104488. https://doi.org/10.1371/journal.pone.0104488
16. Snieder H, Dong Y, Barbeau P, Harshfield GA, Dalageogou C, Zhu H, et al. Beta2-adrenergic receptor gene and resting hemodynamics in European and African American youth. Am J Hypertens. 2002;15(11):973-9. https://doi.org/10.1016/s0895-7061(02)02991-6
17. Weir TD, Mallek N, Sandford AJ, Bai TR, Awadh N, Fitzgerald JM, et al. beta2-Adrenergic receptor haplotypes in mild, moderate and fatal/near fatal asthma. Am J Respir Crit Care Med. 1998;158(3):787-91. https://doi.org/10.1164/ajrccm.158.3.9801035
18. Holloway JW, Dunbar PR, Riley GA, Sawyer GM, Fitzharris PF, Pearce N, et al. Association of beta2-adrenergic receptor polymorphisms with severe asthma. Clin Exp Allergy. 2000;30(8):1097-103. https://doi.org/10.1046/j.1365-2222.2000.00929.x
19. Almomani BA, Al-Eitan LN, Al-Sawalha NA, Samrah SM, Al-Quasmi MN. Association of genetic variants with level of asthma control in the Arab population. Journal of asthma and allergy. 2019:12:35-42. https://doi.org/10.2147/jaa.s186252

Most read articles by the same author(s)